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Sample Requirements

 

All projects, regardless of access pathway, follow the sample requirements listed below.

 

We request more DNA than is required to attempt a study experiment once using manual processing for the following reasons:

 

Optimal results are obtained with high molecular weight genomic DNA. The higher end of the concentration range is recommended for optimal performance for downstream copy number analysis.

 

 

 

 

Sequencing*

Minimum # Experimental Samples

DNA Volume (ul)

Genomic DNA Concentration (ng/ul)

Low Input DNA Volume (ul)**

Low Input DNA Concentration (ng/ul)**

Whole Exome

Not Applicable

50

50

50

5

Custom Targeted

Not Applicable

50

50

50

5

Whole Genome

(PCR - free)

Not Applicable

60

50

Not Applicable

Not

Applicable

*Requirements are for genomic DNA. DNA should be sent in 1XTE, pH 8.0 (10mMTris, 1mM EDTA). Do not use water. Blood, cell line, saliva or FFPE DNA sources are accepted. Please inquire for FFPE requirements.

**Please inquire about additional cost for low input option when combined with our custom targeted sequencing service. There is no additional cost for using our low input option for whole exome sequencing.

 

 

Genotyping

Minimum # Experimental Samples

DNA Volume (ul)

Genomic DNA Concentration (ng/ul)

Whole Genome Amplified DNA Concentration (ng/ul) *

ExomeArray Products

HumanOmni5Exome

46

35

50-100

100-200

 

 

 

 

 

All other Exome inclusive arrays

92

25

50-100

100-200

GWAS Products

Affymetrix Axiom UK BioBank

460

25

50-100

100-200

 

 

 

 

 

HumanOmni2.5

HumanOmniExpress

HumanCore

Global Screening Array

92

25

50-100

100-200

 

 

 

 

 

HumanOmni5

46

35

50-100

100-200

Consortium Developed

Multi-Ethnic Global, AMR/AFR

Multi-Ethnic Global, EUR/EAS/SAS

OncoArray

PsychArray

Drug Core Array

ImmunoArray

92

25

50-100

100-200

Methylation

MethylationEPIC**

540

70

30-50

Not Applicable

Affymetrix Axiom Custom

Custom SNP Product

460

25

50-150

100-200

Illumina Custom iSelect (Infinium)

Custom Panel

1,104

25

50-100

100-200

Linkage

Human Linkage

(Illumina QC Array)

88

25

50-100

100-200

 

 

 

 

 

Mouse Medium Density Linkage Panel

82

25

75-150

200-400

 

 

 

 

 

 

* Whole Genome Amplified (WGA) DNA Information
Based on our experience with the products and services we provide, we discourage the use of WGA samples, especially for Infinium chemistry based assays (GWAS and iSelect custom studies). The ending concentration of WGA samples is not indicative of sample quality.

 

If you do submit WGA samples, you should anticipate a higher non-random missing data rate and perhaps a slightly higher error rate than for genomic DNA. WGA DNA will not give usable results for downstream copy number analysis.

 

For WGA samples, a phi29 based amplification method and higher concentrations are recommended.

 

**Methylation Sample Number Information

Must be in multiples of 90 after minimum number of samples

 

 


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