Investigators have a wealth of study design options available to them using next generation sequencing technologies. We currently offer production scale whole exome and custom targeted sequencing services which utilize LIMS tracking, robotic automation, strict QC standards and automated primary, secondary and tertiary analyses.
Coming in 2012: Whole Genome Sequencing
In most cases, it is expected that all samples will be collected and phenotyped before applying for the NIH CIDR Program. Possible exceptions should be discussed with CIDR and the NIH supporting institute before applying.
Next Generation Sequencing Platform
Illumina HiSeq 2000 sequencers
Run Parameters
Indexed, 75 bp, paired end runs
Enrichment Method
Agilent SureSelect Target Enrichment
Enrichment Size Options
500 kb
Sample Requirements
| Total: | 2.5ug of genomic DNA | |
| Concentration: | 50ng/ul | |
| Diluent: | DNA should be sent in 1XTE, pH 8.0 (10mMTris, 1mM EDTA). Do not use water | |
| Sources: | Blood, cell line or saliva | |
| Buccal swab or WGA samples will not be accepted at this time |
Services Include
Only high coverage sequencing of individual samples are supported. We cannot accommodate the analysis of pooled samples or low coverage study designs at this time.
CNV detection utilizing “counting” methods are also not likely to be highly successful due to the uneven coverage inherent with enrichment chemistry.
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