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Custom Targeted

 

In most cases, it is expected that all samples will be collected and phenotyped before applying for the NIH CIDR Program. Possible exceptions should be discussed with CIDR and the NIH supporting institute before applying.

 

Next Generation Sequencing Platform

     Illumina HiSeq sequencers

 

Run Parameters

     Indexed, 100 bp, paired end runs

 

Enrichment Method

     Agilent SureSelect Target Enrichment

 

Enrichment Size Options

      1.0 - 499 Kb
      0.5 - 2.9 Mb
      3.0 - 5.9 Mb
      6.0 - 11.9 Mb
       12 - 24 Mb
    

Sample Requirements

   Total: 2.25ug of genomic DNA
   Concentration: 30ng/ul (75ul)
   Diluent: DNA should be sent in 1XTE, pH 8.0 (10mMTris, 1mM EDTA). Do not use water
   Sources:Blood, cell line or saliva
    Please inquire about other DNA sources

 

Services Include

 

Only high coverage sequencing of individual samples are supported. We cannot accommodate the analysis of pooled samples or low coverage study designs at this time.

 

CNV detection utilizing “counting” methods are also not likely to be highly successful due to the uneven coverage inherent with enrichment chemistry.

 


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photo of technician

 

Lab Team Coordinator, Nina DiFabion, examines a flowcell